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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > FCM-R5287

Rat FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (MALS & SPR verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (FCM-R5287) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P13599-1(FCGRT) & P07151-1(B2M)).
    Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    Online(Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M)) P13599-1 (FCGRT) & P07151-1 (B2M)

    Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.8 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 43-55 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-R5287) SDS-PAGE gel

Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ) MALS images

The purity of Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-R5287) was more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-R5287) captured on NTA-Series S sensor chip can bind Herceptin® with an affinity constant of 19.9 nM as determined in a SPR assay (Biacore T200) (QC tested).

  • Citations
  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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