Human Serpin D1 / Heparin Cofactor II Protein, His Tag

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Cat. No. / Size
Price
Qty
HC2-H5224-50ug
$300.00
HC2-H5224-1mg (250ug X 4)
$2040.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    SerpinD1, HCF2, HLS2, HC-II

  • Source

    Human Serpin D1, His Tag (HC2-H5224) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Ser 499 (Accession # AAH35028).

    Predicted N-terminus: Gly 20

    Request for sequence
  • Molecular Characterization

    Serpin D1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.7 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Serpin D1 SDS-PAGE

    Human Serpin D1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

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Background

Serpin D1 is also known as Heparin cofactor 2 (HCF2), Protease inhibitor leuserpin-2 (HLS2), Heparin cofactor II (HC-II), which belongs to the serpin family. HCF2 is expressed predominantly in liver. Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, SerpinD1 / HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). In a glycosaminoglycan-independent manner, serpinD1 / HC-II also inhibits chymotrypsin. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

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