Human SIRP gamma / CD172g Protein, Fc Tag

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SIG-H5253-100ug
$260.00
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SIG-H5253-1mg (250ug X 4)
$1,625.00
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SIG-H5253
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Synonyms

SIRPG, CD172g, SIRPB2, SIRP-gamma, SIRP-b2, SIRP-beta-2

Source

Human SIRP gamma, Fc Tag (SIG-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 29 - Ser 364 (Accession # AAH64532).

Predicted N-terminus: Glu 29

Molecular Characterization

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 63.7 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Signal-regulatory protein gamma (SIRPG or SIRP-gamma) is also known as CD172 antigen-like family member B (CD172g), Signal-regulatory protein beta-2 (SIRPB2), is a single-pass type I membrane protein. SIRPG is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG interacts with CD47. On binding with CD47, CD172g mediates cell-cell adhesion.