This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 23.4 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human PD-L2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Captured Human PD-L2 Protein, His Tag (Cat. No. PD2-H5220) on CM5 Chip via anti-His antibody can bind Human PD-1, Fc Tag (Cat. No. PD1-H5257) with an affinity constant of 24.9 nM as determined in SPR assay (Biacore T200) (QC tested).
Loaded Human PD-1 Protein, Fc Tag (Cat. No. PD1-H5257) on Protein A Biosensor, can bind Human PD-L2 Protein, His Tag (Cat. No. PD2-H5220) with an affinity constant of 0.45 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) on Protein A Biosensor, can bind Human PD-L2 Protein, His Tag (Cat. No. PD2-H5220) with an affinity constant of 0.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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