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Your Position: Home > Immune Checkpoint Proteins > PD-L2 > Human B7-DC /PD-L2 /CD273 Protein, with C-Fc Tag

Human PD-L2 / B7-DC Protein, Fc Tag (HPLC-verified)

  • Synonym
    PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2
  • Source
    Human PD-L2, Fc Tag (HPLC-verified) (PD2-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
    Predicted N-terminus: Leu 20
  • Molecular Characterization
    PD-L2(Leu 20 - Pro 219) AAI13679

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) SDS-PAGE gel

Human PD-L2, Fc Tag (HPLC-verified) on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. ) HPLC images

The purity of Human PD-L2, Fc Tag (HPLC-verified)(Cat.# PD2-H5251) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
Human PD-L2, Fc Tag (HPLC-verified)Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) ELISA bioactivity

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 200 μg/mL (100 μL/well) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.156-0.625 μg/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

Flow Cytometry assay shows that recombinant  Human PD-L2, Fc Tag (Cat. No. PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with an EC50 of 0.029 μg/mL (Routinely tested).

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Price(USD) : $1700.00

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