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Your Position: Home > Immune Checkpoint Proteins > OX40 > Human TNFRSF4 /OX40 /CD134 Protein

Human OX40 / TNFRSF4 / CD134 Protein

  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    Human OX40 (OX0-H5224) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # P43489-1).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    OX40(Leu 29 - Ala 216) P43489-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human OX40 (Cat. No. OX0-H5224) SDS-PAGE gel

Human OX40 on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human OX40Human OX40 (Cat. No. OX0-H5224) ELISA bioactivity

Immobilized Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) at 5 μg/mL (100 μL/well) can bind Human OX40 (Cat. No. OX0-H5224) with a linear range of 0.128-2 μg/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant Human OX40 (Cat. No. OX0-H5224)  can bind to 293T cell overexpressing human OX40L. The concentration of OX40 used is 0.3 μg/ml (Routinely tested).

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