My team has been using the Anti-FMC63 Antibody, and they always have consistent and reliable data that is being used for preclinical IND-enabling studies as well as clinical studies. I recommend using AcroBiosystems for your antibody needs.
Our bioanalytical team have consistently obtained reliable CAR detection results using the PE‑labeled monoclonal anti‑FMC63 antibody in our animal studies. The antibody shows strong specificity, clear signal, and low background, with consistent performance across runs. Overall, it has been a dependable, high‑quality reagent, and we consider the vendor a reliable supplier for CAR detection assays.
The FM3-PY54A2 PE antibody from AcroBiosystems has proven to be an outstanding reagent for flow cytometry applications. It delivers exceptional signal clarity and consistency, providing optimal staining and reproducible results across multiple experimental runs. The antibody exhibits high specificity and minimal background, ensuring precise detection and reliable data quality.
Overall, this product demonstrates excellent performance and stability, making it a highly recommended choice for researchers seeking robust and accurate flow cytometry analysis.
ACRO Biosystem's Alexa Fluor™ 647-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) Star Staining is my go-to anti-idiotype product for detection of CD19-targeted synthetic receptors including CARs via flow cytometry. The product stains robustly, with low background signal against non-transduced cells. It was incredibly easy to incorporate this product into my flow cytometry panel. As an added plus, shipping and delivery from ACRO Biosystems was fast and on time.
This antibody demonstrates impressive stability and reliable detection, making it a valuable tool in the lab. Although it is somewhat expensive, its performance matches its cost. It works effectively across a wide range of staining conditions and has shown consistency across multiple cell types. Additionally, I have found that different lots are consistent. Overall, this is an excellent and dependable reagent.
Work in logistics and these reagents were packaged well, delivered in a timely manner, and customer service has been extremely easy and fast to work with. Well organized company!
We have used this biotin anti-FMC63 for detecting our CD19 CAR on the surface of T cells. We use this in combination with a secondary staining with PE anti-Biotin at the recommended dilution and it has been working great. We have purchased multiple batches of this product and it has been consistent form lot to lot. We are happy with this and would recommend this for detecting FMC63.
We work with CD19 CAR T cells with the FMC63 scFv. We use this antibody to detect the surface expression of CD19 CAR on the T cells. We use a secondary PE anti-Biotin antibody following staining with this antibody. The surface staining using this antibody is bright and correlates with the secondary marker GFP we use. We have purchased multiple batches of this product and it has been reliable.
My team purchased the FITC-conjugated Anti-FMC63 antibody (FM3-FY45) to characterize a CAR-T cell line that we had developed. We used the antibody in our flow cytometry experiment, and we saw virtually no background signal in our negative control samples (0% and 1% FITC readout). We did, however, get beautiful results for our CAR-T cell line samples and were able to confirm successful transduction. The instructions for use in the CoA were very clear, and the recommended concentration was sufficient for our purposes. I am very interested in revisiting ACRO Biosystems for more antibody purchases in the future.
everything works super well and there are very little issues that we deal with. it's very simple and easy to use and our scientists are completing their data very well. thank you for the hard work and effort to create easy and reliable products.
We started using this antibody to assess CAR expression in Jurkat cells and it has proven to work perfectly for what we need. We've used it in flow cytomerty anaylsis to great success. It means we can co-stain easily for other markers to ensure our CAR expression is consistent and not lost. Our group has decided this antibody will be our primary method of confirming CAR expression going forward and allows us to determine transduction efficiency easily.
We use this antibody for the selection of CAR cells from the immune cell population. It has been successful with the detection in in-vitro environments with FACS detection. The antibody can accurately select the positive population at a 1:200 dilution with very low background. The antibody is also very stable over long periods of time when it is stored correctly. I would recommend this product to anyone who is looking for a method for detection of FMC63.
I have been using the unconjugated FMC63 for a while and as soon as we knew about the conjugated FMC63 we wanted to use this as it will be saving us 1.5 hours in the staining procedure. We used this antibody and we were able to see a better seperation of positive and negative poulations. Its easy to use and 1:25 dilution works really well. I will recommend this 200%
The PE antibody works very well on our system. We are mainly using it for CAR designing and validation. Our samples are stained with the antibody and tested in multi color flow cytometry in my lab. Good thing is the antibody works very well with our color panel. It also works with other antibodies. The antibody is very stable and we did encounter any problems for preserving or using. The recommended concentration is pretty accurate too. We are happy about this product and definitely coming back for it.
I used this antibody and it worked great! I ordered it for the purpose of detecting CAR expression in T-cells and the antibody was very useful. During analysis with FACS, this Anti-FMC63 monoclonal antibody was great for detecting even low levels of CAR expression and was really easy to use. I would recommend this antibody to anyone having trouble identifying cells that express CAR.
We are very happy with your product as it provides consistent results throughout the products lifetime. We use it for a FACS release panel to evaluate our CAR-T cell therapy and it made our selection after screening dozens of other antibodies. Therefore, we would like to recommend ACRO Biosystems their products for your FACS assays.
We purchase the anti FMC63 to detect Chimeric antigen receptor (CAR) cells in patients treated with an academic CAR for lymphoma. We used the antibody to detect by flow cytometry the cells that express the CAR and the persistence of our modified T cells. We are extremely satisfied with the performance of the antibodies. We are developing new CAR and for sure we will use the antibodies from Acrobiosystems.
The ACROBiosystemsS PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) was purchased for CD19 CAR antigen staining via multi-color flow cytometry at my company. My colleagues and I found that the recommended antibody per sample worked well with our flow assay considering we were using 9 different fluorophores. The antibody has also been stable when reconstituted and sorted in aliquots. Colleagues have recommended that I try this antibody and I would definitely recommend to other colleagues for their assays. I was also very pleased with the quick delivery of the product to enable research to happen.
We are a CAR-T therapy research group and we use FITC anti-FMC63 and FITC-Labeled Human CD19 protein to detect CAR19 cells both in patient blood and in vitro cultures by flow cytometry. The results obtained so far with both reagents are reliable and reproducible, with a clear and easy detection of positive population even when reagents are directly incubated with blood samples.