Save Product
Product Details
Source
Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade is a monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Isotype
Rabbit IgG, Kappa
Application
Flow Cytometry (Evaluation of CAR and SCFV containing 218linker on Human cells).
Specificity
This product is a specific antibody specifically reacts with CAR or scFv containing the 218 linker sequence.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Protein A
<5 ppm of protein tested by ELISA.
Host Cell Protein
<0.5 ng/μg of protein tested by ELISA.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Sterility
Negative
Mycoplasma
Negative
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
For long term storage, the product should be stored at liquid state at -70°C.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 2-8°C for 12 months under sterile conditions;
- -70°C for 24 months.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade (Cat. No. 218-MY23G0) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Report
Bioactivity-FACS
Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade (Cat. No. 218-MY23G0) at 1.6 μg/mL (0.16 μg of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and Goat anti-Rabbit IgG (H+L), PE. PE signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade (Cat. No. 218-MY23G0) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC anti-CD3 antibody and Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09), CF (carrier free), premium grade (0.16 μg of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
