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COADS MSDS

Monoclonal Anti-Human 4-1BB Antibody, Mouse IgG1, premium grade

GMP version GMP-M41B35 is now available for seamless transition.
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COADS MSDS
Cat. No. / Size
Price
Availability
Qty
Premium grade liquid
41B-CH25G1-100ug
$160.00
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41B-CH25G1-1mg (500ug X 2)
$430.00
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GMP liquid
GMP-M41B35-500ug
$1,500.00
In Stock - Delivery in 1-2 business days
GMP-M41B35-2.5mg (500ug X 5)
$5,000.00
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41B-CH25G1
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Features and Advantages
  • Seamless Transition from Preclinical to Clinical: Same cell clone, process, and QC as GMP products.
  • Enhanced Production Standards: AOF raw materials, pharma-grade excipients, Grade B+A (ISO 5) environment.
  • Comprehensive Quality Control: Additional strict testing for process impurities, sterility, and mycoplasma.
  • Cost-Effective Solution: GMP-comparable quality at an economical price for early development.
  • Risk Mitigation: Enhanced safety through rigorous impurity control and comprehensive testing.
Source

Monoclonal Anti-Human 4-1BB Antibody, Mouse IgG1, premium grade (41B-CH25G1) is a chimeric monoclonal antibody recombinantly produced from CHO cells, which combines the variable region of a human monoclonal antibody with mouse IgG1 constant domain.

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
GMP-M41B35 is the GMP version of this 41B-CH25G1. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stage to later clinical phases.

Isotype

Mouse IgG1, Kappa

Specificity

This product is a specific antibody specifically reacts with 4-1BB.

Endotoxin

Less than 0.002 EU per μg by the LAL method / rFC method.

Protein A

<5 ppm of protein tested by ELISA.

Host Cell Protein

<0.5 ng/μg of protein tested by ELISA.

Host Cell DNA

<0.02 ng/μg of protein tested by qPCR.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Sterility

Negative

Mycoplasma

Negative

Formulation

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping

This product is supplied and shipped with dry ice, please inquire the shipping cost.

Storage

For long term storage, the product should be stored at liquid state at -20°C or below.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C or below for 24 months under sterile conditions;
  • 2-8°C for 12 months after thaw if within expiry date.
ACRO Quality Management System
Quality Description

ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.

Background

4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells. Mature human 4-1BB consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine-rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain . Within the ECD, human 4-1BB shares 60% aa sequence identity with mouse and rat 4-1BB. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4, CD8, memory CD8, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts . It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells . This interaction costimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell . It can also enhance the activation-induced cell death of repetitively stimulated T cells . Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells . 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8 T cell proliferation . Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts but supports mature dendritic cell survival and costimulates the proliferation and activation of mast cells.
4-1BB activation enhances CD8 T cell and NK cell mediated anti-tumor immunity . It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome . Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively .