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Product Details
Source
Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) is a non-neutralizing antibody expressed from human HEK293 cells, which provides higher batch consistency and long term security of supply.
Clone
3G8C1
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Recombinant C11D5.3 scFv derived from HEK293 cells
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Specificity
Specifically recognizes C11D5.3 derived CARs.
Application
Flow Cytometry (Evaluation of Anti-BCMA (C11D5.3 scFv) CAR Expression).
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS containing 0.03% ProClin 300, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-FACS
Flow cytometric analysis of Anti-BCMA(C11D5.3) CAR-293 cells staining with Alexa Fluor 647-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-AFMYb) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with Isotype control antibody. Alexa Fluor 647 signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of Alexa Fluor 647-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-AFMYb) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC anti-CD3 antibody and Alexa Fluor 647-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and Alexa Fluor 647 positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
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