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Product Details
Source
Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) is a non-neutralizing mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
3G8C1
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Reactivity
Mouse
Immunogen
Recombinant Mouse C11D5.3 scFv derived from HEK293 cells
Specificity
Specifically recognizes C11D5.3 derived CARs.
Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Liquid
Formulation
Supplied a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized Mouse anti-BCMA scFv (C11D5.3), His Tag at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-C11D5.3 scFv Antibody, Mouse lgG1 (3G8C1) (Carrier-free) (recommended for ADA assay) (Cat. No. C13-MY2010b) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
ELISA analysis shows that the binding of Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) to Monoclonal Anti-Human BCMA Antibody, Human IgG1 was inhibited by increasing concentration of Monoclonal Anti-C11D5.3 scFv Antibody, Mouse lgG1 (3G8C1) (Carrier-free) (recommended for ADA assay) (Cat. No. C13-MY2010b). The concentration of Human BCMA, His Tag, premium grade (Cat. No. BCA-H522y) used is 0.1 μg/mL (100 μL/well) (Routinely tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-C11D5.3 scFv Antibody, Mouse lgG1 (3G8C1) (Carrier-free) (recommended for ADA assay) (Cat. No. C13-MY2010b) captured on CM5 chip via anti-mouse antibodies surface can bind Mouse anti-BCMA scFv (C11D5.3), His Tag with an affinity constant of 62.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Typical Data
Immunohistochemical analysis of paraffin embedded anti-BCMA CAR-T cells labelled with C13-MY2010b.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with lHC staining protocol.
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