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Product Details
Source
Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
Antibody Type
Recombinant Monoclonal
Clone
C1152
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Reactivity
Human
Immunogen
Purified Human CD28 Protein
Conjugate
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
Specificity
This product is a specific antibody specifically reacts with CD28 protein.
Application
Flow Cytometry (Evaluation of the expression of CD28 on Human cells).
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution;
- 2-8 °C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-Flow Cytometry
Flow cytometric analysis of Human peripheral blood lymphocytes respectively staining with APC-Labeled Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 (Cat. No. CD8-AHFC1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of APC-Labeled Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 (Cat. No. CD8-AHFC1) binding to CD3- cells present in human PBMC. Human PBMCs were simultaneously stained with FITC anti-human CD3 Antibody and APC-Labeled Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. FITC negative signals and APC positive signals were used to evaluate the non-specific binding activity to human CD3- cells (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
T-cell-specific surface glycoprotein CD28
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
31 Details
- Highest Development Stage：
Phase 2 Clinical
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