PE-Labeled Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 (0.03% Proclin)

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CD8-PCFC1-50tests
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Source

Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

Antibody Type

Recombinant Monoclonal

Clone

C1152

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Reactivity

Human

Immunogen

Purified Human CD28 Protein

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Specificity

This product is a specific antibody specifically reacts with CD28 protein.

Application

Flow Cytometry (Evaluation of the expression of CD28 on Human cells).

Recommended Dilution

1:50

Isotype Control

The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 12 months after reconstitution.
  • 2-8 °C for 12 months after reconstitution.
Notices

Product Specific Notices: For research use only.

Background

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.