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Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (MALS verified)

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Cat. No. / Size
Price
Qty
CD1-MY2101-100ug
$155.00
CD1-MY2101-500ug
$565.00
ETA of in-stock products:2 business days
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Product Details

  • Source

    Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) is a recombinant mouse monoclonal antibody expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    CD71

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Immunogen

    Recombinant Human CD71 is expressed from human 293 cells

  • Specificity

    Specifically recognizes Human CD71.

  • Application

    Application
    Recommended Usage
    ELISA
    0.2-2 μg/mL
  • Cross Verification

    This product No cross-reactivity in ELISA with
    Rat Transferrin R, His Tag (Cat. No. TFR-R5249).
    Mouse Transferrin R, His Tag (Cat. No. TFR-M524b).

  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     CD71 ELISA

    Immobilized Human Transferrin R, His Tag (Cat. No. CD1-H5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol
  •  CD71 ELISA

    Immobilized Cynomolgus Transferrin R, His Tag (Cat. No. TFR-C524a) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-8 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-SPR

     CD71 SPR

    Captured Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) on CM5 chip via anti-mouse antibodies surface can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 0.821 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.

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