Product Details
Source
Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) is a recombinant mouse monoclonal antibody expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
CD71
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Recombinant Human CD71 is expressed from human 293 cells
Specificity
Specifically recognizes Human CD71.
Application
ApplicationRecommended UsageELISA0.2-2 μg/mLCross Verification
This product No cross-reactivity in ELISA with
Rat Transferrin R, His Tag (Cat. No. TFR-R5249).
Mouse Transferrin R, His Tag (Cat. No. TFR-M524b).Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA

Immobilized Human Transferrin R, His Tag (Cat. No. CD1-H5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus Transferrin R, His Tag (Cat. No. TFR-C524a) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR

Captured Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) on CM5 chip via anti-mouse antibodies surface can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 0.821 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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