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Product Details
Specificity
This product is a specific antibody specifically reacts with DM-1&DM-4.
Source
Biotinylated Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
Isotype
Mouse IgG1 | Mouse Kappa
Conjugate
Biotin
Reactivity
Chemical
Immunogen
DM-1
Application
ApplicationRecommended UsageELISA4-500 ng/mLPurification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA
Immobilized Trastuzumab-DM1 (T-DM1) at 2 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (MALS verified) (Cat. No. DM1-BLY73) with a linear range of 0.98-31.25 ng/mL (QC tested).
Protocol
Immobilized Human Her2 / ErbB2 (498-648) Protein, His Tag (Cat. No. HE2-H52H4) at 2 μg/mL, add increasing concentrations of Trastuzumab-DM1 (T-DM1), and then add Biotinylated Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (MALS verified) (Cat. No. DM1-BLY73) at 0.5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 1.95 ng/mL (Routiney tested).
Protocol
Immobilized ADC-DM4 at 2 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (MALS verified) (Cat. No. DM1-BLY73) with a linear range of 1.95-62.5 ng/mL (Routiney tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Trastuzumab-DM1 in the 100% Human Serum and then add Biotinylated Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (Cat. No. DM1-BLY73) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Trastuzumab-DM1 in the 0.5% BSA and then add HRP conjugated Biotinylated Monoclonal Anti-DM-1&DM-4 Antibody, Mouse IgG1 (Cat. No. DM1-BLY73) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
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