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Product Details
Source
Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1F5) is a rabbit monoclonal antibody recombinantly expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
1M1F5
Isotype
Rabbit IgG, Kappa
Specificity
This product is a specific antibody specifically reacts with Eribulin.
Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1F5) (Cat. No. ERN-MY2062b) at 1 μg/mL, add Farletuzumab Ecteribulin in the 100% Human Serum and then add Biotinylated Human FOLR1, Fc,Avitag (Cat. No. FO1-H82F9) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Farletuzumab Ecteribulin at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1F5) (Cat. No. ERN-MY2062b) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
Serial dilutions of Eribulin were added into Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1F5) (Cat. No. ERN-MY2062b): Farletuzumab ecteribulin binding reactions. The half maximal inhibitory concentration (IC50) is 1.001 μg/mL (Routinely tested).
Protocol
Cross Verification
ELISA binding of Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1F5) (Cat. No. ERN-MY2062b) with Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam, Farletuzumab Ecteribulin, IgG1-MMAF and Trastuzumab-DM1 conjugated antibody respectively.
The coating antibody was Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1F5) (Cat. No. ERN-MY2062b), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam, Farletuzumab Ecteribulin, IgG1-MMAF and Trastuzumab-DM1 conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-Eribulin Antibody, Rabbit IgG (1M1F5) (Cat. No. ERN-MY2062b) is specific to Farletuzumab Ecteribulin, and has no cross-reactivity with Trastuzumab Deruxtecan, Sacituzumab Govitecam, Disitamab Vedotin (RC48), IgG1-MMAF and Trastuzumab-DM1 (Routinely tested).
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