Monoclonal Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F)

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Cat. No. / Size
Price
Qty
RSF-M309a-100ug
$415.00
RSF-M309a-1mg
$2735.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) is a recombinant mouse monoclonal antibody expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    101F

  • Isotype

    Mouse IgG2a, Kappa

  • Host Species

    Mouse

  • Specificity

    This product is a specific antibody specifically reacts with RSV-F0.

  • Application

    Application
    Recommended Usage
    ELISA
    0.2-2 μg/mL
  • Cross Verification

    This product can cross in Western Blot with
    HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6).
    HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7).
    HRSV (B) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H9).
    HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8).

  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Fusion glycoprotein F0/F protein (RSV) ELISA

    Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6) at 2 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) (Cat. No. RSF-M309a) with a linear range of 0.2-10 ng/mL (QC tested).

    Protocol
  •  Fusion glycoprotein F0/F protein (RSV) ELISA

    Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) (Cat. No. RSF-M309a) with a linear range of 0.2-10 ng/mL (QC tested).

    Protocol

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Background

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation. Antibody 101F, target epitopes that have been mapped to linear regions in the F1 subunit, referred to as antigenic site IV.

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