Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) (MALS verified)

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Cat. No. / Size
Price
Qty
GL2-MY2095-100ug
$415.00
GL2-MY2095-1mg (500ug X 2)
$2735.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    4D1

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    rubella virus (strain M33) (RUBV)

  • Immunogen

    Recombinant Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV) is expressed from Baculovirus-Insect cells

  • Specificity

    Specifically recognizes Rubella virus Glycoprotein E1 Protein.

  • Application

    Application
    Recommended Usage
    Western Blot
    10 ug/mL
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Glycoprotein E2 & E1 ELISA

    Immobilized Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV), His Tag&Strep II Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) (Cat. No. GL2-MY2095) with a linear range of 0.3-5 ng/mL (QC tested).

    Protocol
  • Western Blot

     Glycoprotein E2 & E1 WESTERN BLOT
  • Detection of Monoclonal Anti-Rubella virus Glycoprotein E2 & E1(GL2-R5583) antibody-4D1, Human IgG1 | Human Kappa ,HEK by Western Blot. Monoclonal Anti-Rubella virus Glycoprotein E2 & E1(GL2-R5583) antibody-4D1, Human IgG1 | Human Kappa, HEK at 10 ug/ml dilution + Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV), His Tag&Strep II Tag (MALS verified), His Tag at 400ng.<br>
    Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
    Predicted band size: 95 kDa 12% Bis-Tris Protein Gel.

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Background

Rubella virus (RV), the etiological agent of German measles, is a small enveloped RNA virus that belongs to the togavirus family. RV virions contain two glycosylated membrane proteins, E1 and E2, that exist as a heterodimer and form the viral spike complexes on the virion surface. Formation of an E1-E2 heterodimer is required for transport of E1 out of the endoplasmic reticulum lumen to the Golgi apparatus and plasma membrane.

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