Product Details
Source
Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Human IgG1 (6D1) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
Clone
6D1
Species
Mouse
Isotype
Human IgG1 | Human Kappa
Conjugate
Unconjugated
Antibody Type
Recombinant Monoclonal
Reactivity
Virus
Immunogen
Recombinant Influenza A [A/Wisconsin/588/2019 (H1N1)] HA derivedfrom human 293 cells
Specificity
This product is a specific antibody specifically reacts with Hemagglutinin/HA (Influenza Virus).
Application
ApplicationRecommended UsageELISA0.1-50 ng/mLPurity
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cross Verification
This product can cross in Elisa with
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H14).
No cross-reactivity in ELISA with
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No.HA2-V52H6).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5).
Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4).ACRO Quality Management System
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Performance Data
SDS-PAGE

Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Human IgG1 (6D1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Human IgG1 (6D1) (Cat. No. HA1-M648) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Human IgG1 (6D1) (Cat. No. HA1-M648) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Immobilized Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Human IgG1 (6D1) (Cat. No. HA1-M648) with a linear range of 0.5-8 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR

Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Human IgG1 (6D1) (Cat. No. HA1-M648) captured on Protein A Chip can bind Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3) with an affinity constant of 0.169 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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