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Application
The Monoclonal Anti-His Tag Antibody, Mouse IgG1 FA-Labeled is a universal tool for TR-FRET assays that can bind His-tagged proteins. This product uses specific anti-His antibody labeled with fluorescent dyes and it can be used in combination with other Donors covalently conjugated with Eu³⁺ chelate. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.Product Specifications
SourceThis Monoclonal Anti-His Tag Antibody, Mouse IgG1 is a mouse monoclonal antibody recombinantly expressed from Hybridoma.SpecificityThis antibody specifically reacts with His tag proteins, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the his-tagged protein as acceptor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Performance Data
Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Mouse IgG1 FA-Labeled (Cat. No. FRT-TA05) is used as the Acceptor. Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1), at 12.5 ng/well, can bind to the Human PD-L1, His Tag (Cat. No. PD1-H5229) with a linear detection range of 10-0.02 μg/mL (QC tested).
In this TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Mouse IgG1 FA-Labeled (Cat. No. FRT-TA05) is used as the Acceptor. An inhibition Assay was performed to evaluate the interaction between Biotinylated Human PD-1, Fc, Avi tag (Cat. No. PD1-H82F1) and Human PD-L1, His Tag (Cat. No. PD1-H5229) using Monoclonal Anti-Human PD-1 Antibody, Human IgG4 resulting in a typical IC50 of 1.774 μg/mL (Routinely tested).
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Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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