Alexa Fluor™ 488-Labeled Monoclonal Anti-His Antibody, Human IgG1 (55F8)

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Cat. No. / Size
Price
Qty
HIS-AFM549-25tests
$73.00
HIS-AFM549-200tests
$309.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-His Antibody, Human IgG1 (55F8) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain, which provides higher batch consistency and long term security of supply.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    55F8

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    His tag (N‑terminal, C‑terminal, internal His‑tagged fusion proteins)

  • Immunogen

    A protein peptide with his tag

  • Conjugate

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • Specificity

    This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

  • Application

    Flow Cytometry (Detection the His-tagged protein).

  • Recommended Dilution

    1:50

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     His FACS

    Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with 100 μL of 10 μg/mL Human CD19 (20-291) Protein, His Tag (Cat. No. CD9-H52H2) and negative control protein respectively, washed and then followed with Alexa Fluor 488-Labeled Monoclonal Anti-His Antibody, Human IgG1 (55F8) (Cat. No. HIS-AFM549) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and analyzed with FACS. Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.

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