FITC-Labeled Human IgG1 Kappa Isotype Control (FITC, mAb, carrier free)

Source

Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. FITC-Labeled Human IgG1 Kappa Isotype Control (mAb) was purified from HEK293 cell culture. It is the FITC labeled form of Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2).

Application

This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.

Species

Human

Isotype

Human IgG1 | Human Kappa

Specificity

Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 12 months under sterile conditions after reconstitution.

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