Biotinylated Mouse IgG1 Isotype Control (Biotin, mAb, carrier free)

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DNP-BM1-25ug
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DNP-BM1-200ug
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Source

Biotinylated Mouse IgG1 Isotype Control (mAb) is a recombinant mouse monoclonal antibody expressed in HEK293 cells.

Antibody Type

Recombinant Monoclonal

Clone

AM1

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Conjugate

Biotinylated

Specificity

Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.

Application
Application
Recommended Use
ELISA
1:200-1:2000

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Purification

Protein A purified.

Aggregation

Less than 5%, as determined by SEC-MALS.

Concentration

Please refer to the Certificate of Analysis (CoA).

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
Notices

Product Specific Notices: For research use only.

Background

Fluorescently or biotin labeled isotype controls are used to assess non specific staining and autofluorescence in flow cytometry, ELISA, and imaging assays. By recognizing DNP, a hapten absent in biological samples, these controls provide a reliable baseline to distinguish background fluorescence or signal from true antigen specific binding.