Alexa Fluor™ 555-Labeled Rabbit IgG Antibody Isotype Control (0.03% Proclin)

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IGG-AEFM776-25tests
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Source

Monoclonal Rabbit IgG Antibody Isotype Control is a chimeric monoclonal antibody recombinantly expressed in HEK293 cells, which combines the variable region of a mouse monoclonal antibody with rabbit IgG constant domain.

Antibody Type

Recombinant Monoclonal

Clone

AM776

Isotype

Rabbit IgG, Kappa

Host Species

Mouse

Immunogen

DNP (Dinitrophenyl)

Conjugate

AF555

Excitation Wavelength: 561 nm

Emission Wavelength: 572 nm

Specificity

Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins, which is normally not present in Humans or animals. Therefore, IGG-AEFM776 does not react with any antigen of Human or animal cells.

Application

Flow Cytometry.

Recommended Dilution

1:50

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months after reconstitution.
  • 2-8 °C for 12 months after reconstitution.
Notices

Product Specific Notices: For research use only.

Background

Fluorescently or biotin labeled isotype controls are used to assess non specific staining and autofluorescence in flow cytometry, ELISA, and imaging assays. By recognizing DNP, a hapten absent in biological samples, these controls provide a reliable baseline to distinguish background fluorescence or signal from true antigen specific binding.