Recombinant Monoclonal Anti-Ki-67 Antibody, Rabbit (7A4)

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Source

Recombinant Monoclonal Anti-Ki-67 Antibody, Rabbit (7A4) is a Ki-67 primary antibody for IHC application.

Antibody Type

Recombinant Monoclonal

Clone

7A4

Isotype

Rabbit IgG, Rabbit Kappa

Host Species

Rabbit

Reactivity

Human

Specificity

React with Ki-67 protein on the intracellular area of target cells.

Application
Application
Recommended Use
IHC-P
1: 200 - 1: 1000

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Purification

Protein A purified.

Concentration

0.9~1.1 mg/ml

Form

Liquid

Formulation

Supplied a 0.22 μm-filtered solution in Tris buffer containing 1% BSA.

Please contact us for customized product forms or formulations.

Shipping

Liquid product is shipped at blue ice.

Storage

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • 2°C to 8°C for 14 days;
  • -20°C to -70°C for 12 months.
Notices

Product Specific Notices: For research use only.

Background

Ki-67 is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.