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Product Details
Source
Biotinylated Monoclonal Anti-MMAE & MMAF Antibody, Mouse IgG1 is a recombinant mouse monoclonal antibody expressed in HEK293 cells and subsequently biotinylated.
Antibody Type
Recombinant Monoclonal
Isotype
Mouse IgG1, Kappa
Immunogen
MMAE
Conjugate
Biotin
Specificity
Specifically recognizes the target-MMAE&MMAF.
Application
ApplicationRecommended UsageELISA1:200-1:2000Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized Disitamab Vedotin (RC48) at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Disitamab Vedotin (RC48) with a linear range of 0.0078-0.125 ng/mL (Routinely tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Disitamab Vedotin (RC48) in the 0.5% BSA and then add Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
Immobilized Human IgG1 Kappa Isotype Control (mAb)-MMAF at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) with a linear range of 0.5-31 ng/mL (Routinely tested).
Protocol
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