Product Details
Source
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1G04) is a recombinant rabbit monoclonal antibody expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
M1G04
Isotype
Rabbit IgG, Kappa
Immunogen
MMAE-BSA
Specificity
Specifically recognizes MMAE and does not recognize MMAF.
Application
ApplicationRecommended UsageELISA1:10000-1:100000Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA

Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1G04) (Cat. No. MME-MY2210) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1G04) (Cat. No. MME-MY2210) with a linear range of 0.06-4 ng/mL (Routinely tested).
Protocol
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1G04) (Cat. No. MME-MY2210) at 5 μg/mL, add Disitamab Vedotin (RC48) and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
Cross Verification

ELISA binding of Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1G04) (Cat. No. MME-MY2210) with Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibody respectively.
The coating antibody was Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1G04) (Cat. No. MME-MY2210), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1G04) (Cat. No. MME-MY2210) is specific to Disitamab Vedotin (RC48) and Trastuzumab MMAE, and has no cross-reactivity with Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 (Routinely tested).
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