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Product Details
Specificity
Specifically recognizes the target-MMAE&MMAF.
Source
Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
Isotype
Mouse IgG1 | Mouse kappa
Conjugate
Biotin
Reactivity
Chemical
Immunogen
MMAE
Application
ApplicationRecommended UsageELISA2-250 ng/mLPurification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA
Immobilized Disitamab Vedotin (RC48) at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Disitamab Vedotin (RC48) with a linear range of 0.0078-0.125 ng/mL (Routinely tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL, add Disitamab Vedotin (RC48) in the 0.5% BSA and then add Biotinylated Monoclonal Anti-MMAE&MMAF Antibody, Mouse IgG1 (Cat. No. MME-BLS104) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
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