Product Details
Source
Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS41) (NUN-S41) is isolated from a SARS-CoV-2 infected patient and is recombinantly produced from human 293 cells (HEK293). As verified by binding test with N-NTD (Cat. No. NUN-C5143) and N-CTD (Cat. No. NUN-C5145) protein, this antibody can only bind to N-CTD (AA Ser 255 - Pro 364). ELISA test validated that this antibody can bind multiple N protein variants (Cat. No. NUN-C52H8) with similar affinity as compared to the wild type N protein (Cat. No. NUN-C5227).
Clone
AS41
Isotype
Human IgG1 | Kappa
Antibody Type
Recombinant Monoclonal
Reactivity
Virus
Specificity
This product can recognize SARS-CoV-2 and SARS-CoV Nucleocapsid protein. No cross-reactivity is detected with nucleocapsid protein of other coronaviruses, including MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
Application
ApplicationRecommended UsageELISA0.1-10 ng/mLPurity
95% as determined by SDS-PAGE.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS41) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS41) (Cat. No. NUN-S41) with a linear range of 0.08-1 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS41) (Cat. No. NUN-S41) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid protein, His Tag (B.1.1.529/Omicron) (Cat. No. NUN-C52Ht) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
Nucleocapsid proteins
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
1 Details
- Highest Development Stage:
Phase 1 Clinical
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