Biotinylated Monoclonal Anti-PBD Antibody, Rabbit IgG (1M1F9)

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Cat. No. / Size
Price
Qty
PBD-BLMY2212-100ug
$370.00
PBD-BLMY2212-1mg (500ug X 2)
$2388.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Biotinylated Monoclonal Anti-PBD Antibody, Rabbit IgG (1M1F9) is a rabbit monoclonal antibody recombinantly expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    1M1F9

  • Isotype

    Rabbit IgG, Kappa

  • Immunogen

    PBD-BSA

  • Conjugate

    Biotin

  • Specificity

    Specifically recognizes PBD.

  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     PBD ELISA

    Immobilized Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) at 5 μg/mL, add Loncastuximab tesirine in the 100% Human Serum and then add Biotinylated Monoclonal Anti-PBD Antibody, Rabbit IgG (1M1F9) (Cat. No. PBD-BLMY2212) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).

    Protocol

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Background

Pyrrolobenzodiazepine (PBD) dimer, is a new generation of cytotoxic payload used in antibody-drug conjugates (ADCs). The PBD dimer binds to the minor groove of DNA to form effective cytotoxic DNA interstrand crosslinks, which can block cell division and kill cancer cells. This mechanism of action utilizes a completely different cellular target from that of tubulin inhibitors, as well as a different DNA damage pattern from other DNA-targeting payloads. Anti-PBD antibody is a rabbit monoclonal antibody specially reacts with PBD, which is more sensitive than mouse antibody. The anti-PBD antibody is a useful reagent in PK assay to determine conjugated antibodies.

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