Biotinylated Monoclonal Anti-PBD Antibody, Rabbit IgG (M1D08)

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Cat. No. / Size
Price
Availability
Qty
PBD-BLMY2221-100ug
$370.00
In Stock - Delivery in 2-3 business days
PBD-BLMY2221-1mg (200ug X 5)
$2388.00
In Stock - Delivery in 2 weeks

Product Details

  • Source

    Chimeric Biotinylated Monoclonal Anti-PBD Antibody, Rabbit IgG (M1D08) is recombinantly expressed in HEK293. It consists of rabbit variable regions genetically fused to rabbit IgG constant domains.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    M1D08

  • Isotype

    Rabbit IgG, Kappa

  • Host Species

    Rabbit

  • Immunogen

    PBD-BSA

  • Conjugate

    Biotinylated

  • Specificity

    Specifically recognizes PBD.

  • Application

    Application
    Recommended Use / Performance
    ELISA
    1:300-1:3000
  • Purification

    Protein A purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     PBD ELISA

    Immobilized Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) at 5 μg/mL, add Loncastuximab tesirine in the 100% Human Serum and then add Biotinylated Monoclonal Anti-PBD Antibody, Rabbit IgG (M1D08) (Cat. No. PBD-BLMY2221) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).

    Protocol

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FAQ

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Background

Pyrrolobenzodiazepine (PBD) dimer, is a new generation of cytotoxic payload used in antibody-drug conjugates (ADCs). The PBD dimer binds to the minor groove of DNA to form effective cytotoxic DNA interstrand crosslinks, which can block cell division and kill cancer cells. This mechanism of action utilizes a completely different cellular target from that of tubulin inhibitors, as well as a different DNA damage pattern from other DNA-targeting payloads. Anti-PBD antibody is a rabbit monoclonal antibody specially reacts with PBD, which is more sensitive than mouse antibody. The anti-PBD antibody is a useful reagent in PK assay to determine conjugated antibodies.

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