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Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59)

COADS MS
DS

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Cat. No. / Size
Price
Qty
S1N-S59A1-100ug
$450.00
S1N-S59A1-1mg
$3255.00
ETA of in-stock products:2 business days
Sub-Total$ 0
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Product Details

  • Source

    Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) (S1N-S59A1), originally from mouse immunized with recombinant SARS-CoV-2 Spike S1 protein, is produced from hybridoma.

  • Clone

    AS59

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Virus

  • Specificity

    This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.

  • Application

    Application
    Recommended Usage
    ELISA
    0.1-10 ng/mL

  • Purity

    95% as determined by SDS-PAGE.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Spike S1 SDS-PAGE

    Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

    Spike S1 ELISA

    Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H1) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) (Cat. No. S1N-S59A1) with a linear range of 0.08-3 ng/mL (QC tested).

    Protocol

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Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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