Human EGFR & LGR5 (SRE-Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human EGFR & LGR5 (SRE-Luc) HEK293 Reporter Cell expressing human EGFR was engineered to not only express SRE signaling response element, but also express the receptor human LGR5 (Uniprot: O75473-1). When stimulated with human EGF protein, receptor-mediated signaling can drive SRE-mediated luminescence. Neutralization of biological effect of the ligand-receptor interaction by either ant-human EGFR antibody or anti-human EGFR × LGR5 bispecific antibody results in a decrease in luminescence, with the overexpressed LGR5 exerting a further enhancement effect.

• Screen for anti-human EGFR × LGR5 bispecific antibody.

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.