Human IL-7 R alpha/CD132 (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human IL-7 R alpha/CD132 (Luc) HEK293 Reporter Cell was engineered to not only express STAT5 signaling response element, but also express the receptors full length human IL-7 R alpha (Uniprot: P16871-1) and CD132 (Uniprot: P31785-1). When stimulated with human IL-7 protein, the IL-7 R alpha/CD132 interaction drives STAT5-mediated luminescence. Neutralization of biological effect of human IL-7 protein by corresponding antibody results in a decrease in luminescence.

• Screen for neutralizing antibodies blocking the stimulation of human IL-7 protein

• Bioactivity detection of human IL-7 fusion protein

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) + G418 (200 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.