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Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteDLL4Format96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity< 18.75 pg/mLStandard Curve Range18.75 pg/mL-1200 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination of human DLL4 in Cell Culture Supernatants, Plasma, Serum.Sample volume100 μLProduct Overview
Human DLL4 ELISA Kit (Residue Testing) is based on the ELISA indirect method and is used to detect and quantitatively determine GMP human DLL4 residues in cell culture supernatants, serum, and plasma. The kit contains GMP Human DLL4 Protein, Fc Tag (ACROBiosystems, cat# GMP-DL4H28) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of human DLL4.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
Unopened kit should be stored at 2°C -8°C upon receiving.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES052-C01Pre-coated Anti-DLL4 Antibody Microplate1 plate (8×12 strips)RES052-C02Human Fc Tag DLL4 Standard20 μgRES052-C03Biotin-Anti-DLL4 Antibody150 μLRES052-C04Streptavidin-HRP50 μLRES052-C0510xWashing Buffer50 mLRES052-C062xDilution Buffer50 mLRES052-C07Substrate Solution12 mLRES052-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Fc Tag DLL4 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Intra-Assay Statistics
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
Recovery
Three parts of blank serum were added with different concentrations of human Fc Tag DLL4, and the serum without human Fc Tag DLL4 was used as background to calculate the recovery rate. The range of the recovery rate is 83.7-116.5%, and the average recovery is 98.6%.
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Drug Development Progress- English Name：
Delta-like protein 4
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 3 Clinical
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