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Product Details
Product Overview
The 293T resDNA Quantitation Kit is designed for the quantitative detection of residual 293T host cell DNA in biomanufacturing processes that employ the 293T cell line, such as the production of viral vectors for gene therapy, CAR-T cell products, recombinant proteins, antibodies, and viral vaccines. Based on real-time quantitative PCR (qPCR) technology, this kit delivers sensitive, specific, and reproducible quantification of residual 293T DNA with a lower limit of quantitation (LLOQ) of 3 fg/µL. This kit is applied after host cell DNA isolation from test samples. For improved DNA recovery and consistent performance, it is recommended to use the kit together with the HEK293 / HEK293T resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R033).
Features
- Ultra-high sensitivity for optimal product safety: Stable detection of HEK293T host cell residual DNA down to 3 fg/μL
- High specificity: No cross-reactivity with unrelated DNA
- Regulatory compliance: ICH Q2(R2) & FDA Bioanalytical Method Validation compliant
- Premium quality: Manufactured in ISO 13485 certified facility
Application
The kit is used for quantitative detection of residual Human DNA in biopharmaceuticals.
For use in quality control/manufacturing process only.
It is for research use only.
Technical Specifications
Attention
If your experimental workflow involves host cell DNA sample preparation, please use the recommended sample preparation kits listed in the table. This ensures that the buffers used during both sample preparation and residual DNA quantitation remain consistent, supporting reliable and accurate results.
Materials Provided
IDComponentsSizeOPA-R032-012×qPCR Master Mix1.0 mL×2OPA-R032-02HEK293T Primer & Probe Mix700 μLOPA-R032-03HEK293T DNA Control（3ng/μL）100 μLOPA-R032-04Dilution Buffer1.5 mL×3OPA-R032-05DNase/RNase-Free Water1.0 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
Figure 1. High sensitivity and broad dynamic range of the HEK293T resDNA Ultra-Low Quantitation Kit. (A) Representative analysis results for Standards 1 (300 pg/μL) through 6 (3 fg/μL). (B) Standard curve generated from the 10-fold serial dilution series. The expected PCR efficiency is 90-110%.
Figure 2. Assay specificity. Standard curves were generated using a 10-fold serial dilution of HEK293T genomic DNA (included in the kit) in the presence of CHO, E. coli, and Pichia pastoris DNA.
Compatibility with a Broad Range of DNA Fragment Sizes
HEK293T genomic DNA was fragmented by enzymatic digestion for 10, 20, and 30 minutes, generating DNA of varying fragment sizes. Each fragmented DNA sample was subjected to 10-fold serial dilution and quantified using qPCR. The resulting standard curves for all fragment sizes met acceptance criteria, indicating that DNA fragmentation has no significant impact on the assay performance.
Figure 3. Standard Curves for HEK293T DNA with Different Fragmentation Levels
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