Human IL-6 ELISA Kit

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CRS-B001-96tests
$565.00
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CRS-B001-480tests (96tests X 5)
$2,545.00
CRS-B001-960tests (96tests X 10)
$4,520.00
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Product Overview

Human IL-6 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-6 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

  • Standards to calibrate with NIBSC/WHO standards for comparable results.
  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
IL-6
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Human
Sensitivity
2.0 pg/mL
Assay Time
1.75 h
Sample volume
50 μL
Range
6.25 pg/mL-400 pg/mL
Sample Type
Cell Culture Supernatants, Plasma, Serum.
NIBSC Code
89/548

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

Application

The kit is developed for quantitative detection of natural and recombinant human IL-6 in serum, plasma and cell culture supernatants.

It is for research use only.

Workflow

IL-6 Workflow

Storage

Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CRB001-C01
Pre-coated Anti-IL-6 Antibody Microplate
1 plate
CRB001-C02
Human IL-6 Standard
20 μg×2
CRB001-C03
Biotin-Anti-IL-6 Antibody Con. Solution
100 μL
CRB001-C04
Biotin-Antibody Dilution Buffer
8 mL
CRB001-C05
Streptavidin-HRP Con. Solution
500 μL
CRB001-C06
Streptavidin-HRP Dilution Buffer
15 mL
CRB001-C07
20×Washing Buffer
50 mL
CRB001-C08
1×Dilution Buffer
15 mL×2
CRB001-C09
Substrate Solution
12 mL
CRB001-C10
Stop Solution
6 mL