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Product Details
Materials Provided
IDComponentsSizeEP146-C01High-bind Plate1 plateEP146-C02Human IL-6 R alpha15 μgEP146-C03Biotinylated Human IL-610 μgEP146-C04Anti-IL-6 Neutralizing Antibody10 μgEP146-C05Streptavidin-HRP10 μgEP146-C06Coating Buffer12 mLEP146-C0710xWashing Buffer50 mLEP146-C08Blocking Buffer50 mLEP146-C09Substrate Solution12 mLEP146-C10Stop Solution7 mLProduct Overview
IL-6 [Biotinylated] : IL-6 R alpha Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human IL-6binding to human IL-6 R. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human IL-6binding to human IL-6 R.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF IL-6 [Biotinylated]: IL-6 R alpha BINDING BY ANTI-IL6 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-6 Neutralizing antibody (Catalog # EP146-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated IL-6: IL-6 R alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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Drug Development Progress- English Name：
Interleukin-6
- Category：
- Approved Drugs：
5 Details
- Drugs in Clinical Trials：
22 Details
- Highest Development Stage：
Approved
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