Human Insulin ELISA Kit

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CEA-B205-96tests
$515.00
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Product Overview

Human Insulin ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human insulin that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

  • Standards to calibrate with NIBSC/WHO standards for comparable results.
  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
Insulin
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Human
Sensitivity
4.69 μIU/mL
Assay Time
1.75 h
Sample volume
50 μL
Range
4.69 μIU/mL-300 μIU/mL
Sample Type
Cell Culture Supernatants, Plasma, Serum.
NIBSC Code
11/212

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Application

The kit is developed for quantitative detection of natural and recombinant human insulin in serum, plasma and cell culture supernatants.

It is for research use only.

Workflow

Insulin Workflow

Storage

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CEA205-C01
Pre-coated Anti-InsμLin Antibody Microplate
1 plate
CEA205-C02
Human InsμLin Standard (Std.-1)
300 μIU/mL
CEA205-C03
Biotin-Anti-InsμLin Antibody Con. Solution
400 μL
CEA205-C04
Biotin-Antibody Dilution Buffer
8 mL
CEA205-C05
Streptavidin-HRP Con. Solution
500 μL
CEA205-C06
Streptavidin-HRP Dilution Buffer
15 mL
CEA205-C07
20×Washing Buffer
50 mL
CEA205-C08
Sample Dilution Buffer
15 mL×2
CEA205-C09
Substrate Solution
12 mL
CEA205-C10
Stop Solution
6 mL