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Product Details
FAB
- Direct labeling - Eliminates secondary antibodies for a more direct signal, smaller molecular weight, and greater analytical flexibility.
- High signal to noise ratio - Strong fluorescence with minimal background.
- pH sensitive - Bright emission in acidic milieu.
- Rapid fluorescent labeling - Only 30 minutes of hands-on time.
- Broad antibody compatibility - Labeling via antibody amines works across different antibody species and subtypes.
Materials Provided
BoxA(-20°C)IDComponentsSizeALK-A004-C01Activated pH-Sensitive Fluorescent Dye (Em660)1 vialｘ3ALK-A004-C021M NaHCO31 vialALK-A004-C03Quencher Reagent1 vialBoxB(4°C)IDComponentsSizeALK-A004-1-C011xPBS (pH7.2-7.4), 11% Trehalose5 mLALK-A004-1-C02Desalting Spin column 0.5 mL3 perProduct Overview
The pH-sensitive dye (Em660) cannot only significantly enhance the fluorescence signal in an acidic environment but also minimize background noise effectively, which has an excitation wavelength of 643 nm and an emission wavelength of 660 nm.
pH-Sensitive Fluorescent Dye Antibody Labeling Kit provides a rapid and simple method to covalently label antibodies with pH-sensitive fluorescent dye by targeting primary amine groups.
Application
pH-sensitive Dye Antibody Labeling Kit (Em660) provides a rapid and simple method for achieving covalent coupling between pH-sensitive fluorescent dye and antibody molecules. The conjugation reaction requires simple purification via spin column chromatography with straightforward operation, and the prepared dye-labeled antibody can be used for the detection of antibody internalization efficiency in cells.
It is for research use only.
Storage
The transport temperature for the kit is 2-8°C.
• BoxA: Unopened kit should be stored at -20℃ upon receiving.
• BoxB: Unopened kit should be stored at 2-8℃ upon receiving.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
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Performance Data
Bioactivity-FACS
Please refer to DS document for the assay protocol.
Anti-Her2 Ab and Human IgG1 isotype control were labeled using pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Cat. No. ALK-A004). SK-BR-3 cells were treated with Dye Labeled Anti-Her2 Ab and Dye Labeled Isotype Control separately at 37℃ for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the internalization signal.
Anti-CD20 Ab and Human IgG1 isotype control were labeled using pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Cat. No. ALK-A004). Raji cells were treated with Dye Labeled Anti-CD20 Ab and Dye Labeled Isotype Control separately at 37℃ for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the internalization signal.
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