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Assay Type | FACS |
Analyte | IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2 |
Format | 96T |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | 1.77 pg/ml (IFN-γ), 0.76 pg/ml (TNF-α), 0.27 pg/ml (IL-10), 0.78 pg/ml (IL-6), 0.65 pg/ml (IL-4), 0.70 pg/ml (IL-2) |
Standard Curve Range | 9.8-10000 pg/mL |
Assay Time | 3 h |
Suitable Sample Type | Human serum, Human plasma, Cell culture supernatants |
Sample volume | 10 μL |
T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized-some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.
It is for research use only.
The unopened kit is stable for 18 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
FCM05-C01 | Calibrator | 5 μg（for each）×2 |
FCM05-C02A | Human IL-2 Capture Beads | 96 tests |
FCM05-C02B | Human IL-4 Capture Beads | 96 tests |
FCM05-C02C | Human IL-6 Capture Beads | 96 tests |
FCM05-C02D | Human IL-10 Capture Beads | 96 tests |
FCM05-C02E | Human TNF-α Capture Beads | 96 tests |
FCM05-C02F | Human IFN-γ Capture Beads | 96 tests |
FCM05-C03 | 2×Assay Buffer | 40 mL |
FCM05-C04 | 10×Wash Buffer | 10 mL |
FCM05-C05 | Detection antibody | 0.5 mL |
FCM05-C06 | 96-well V-bottom plate | 1 plate |
FCM05-C07 | 96-well Sealing film | 2 pieces |
FCM05-C08 | APC Positive Control | 0.5 mL |
FCM05-C09 | PE Positive Control | 0.5 mL |
Human Th1/Th2 Cytokine 6-plex panel is a multiplex bead-based indirect immunofluorescence assay, utilizing flow cytometry for data acquisition and classification. The beads conjugated with specific antibody, target protein (IL-2/4/6/10, TNF-α, IFN-γ) in specimens and PE-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. The intensity of PE fluorescence signal in proportion to the target protein in specimens, are assessed by flow cytometry.The present assay has been optimized for quntitatively analysis for the IL-2/4/6/10, TNF-α, IFN-γ in cell culture supernatant, plasma, and serum samples.