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Product Details
Product Specifications
Assay TypeFACSAnalyteIFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2Format96TReactivityHumanRegulatory StatusRUOSensitivity1.77 pg/ml (IFN-γ), 0.76 pg/ml (TNF-α), 0.27 pg/ml (IL-10), 0.78 pg/ml (IL-6), 0.65 pg/ml (IL-4), 0.70 pg/ml (IL-2)Standard Curve Range9.8-10000 pg/mLAssay Time3 hrSuitable Sample TypeHuman serum, Human plasma, Cell culture supernatantsSample volume10 μLProduct Overview
T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized-some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
Application
The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.
It is for research use only.
Storage
The unopened kit is stable for 18 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeFCM05-C01Calibrator5 μg（for each）×2FCM05-C02AHuman IL-2 Capture Beads96 testsFCM05-C02BHuman IL-4 Capture Beads96 testsFCM05-C02CHuman IL-6 Capture Beads96 testsFCM05-C02DHuman IL-10 Capture Beads96 testsFCM05-C02EHuman TNF-α Capture Beads96 testsFCM05-C02FHuman IFN-γ Capture Beads96 testsFCM05-C032×Assay Buffer40 mLFCM05-C0410×Wash Buffer10 mLFCM05-C05Detection antibody0.5 mLFCM05-C0696-well V-bottom plate1 plateFCM05-C0796-well Sealing film2 piecesFCM05-C08APC Positive Control0.5 mLFCM05-C09PE Positive Control0.5 mLAssay Principles
Human Th1/Th2 Cytokine 6-plex panel is a multiplex bead-based indirect immunofluorescence assay, utilizing flow cytometry for data acquisition and classification. The beads conjugated with specific antibody, target protein (IL-2/4/6/10, TNF-α, IFN-γ) in specimens and PE-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. The intensity of PE fluorescence signal in proportion to the target protein in specimens, are assessed by flow cytometry.The present assay has been optimized for quntitatively analysis for the IL-2/4/6/10, TNF-α, IFN-γ in cell culture supernatant, plasma, and serum samples.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
A double logarithmic fitting curve was plotted for each cytokine( IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ). The present curve of each cytokines was ranging from 9.8 pg/ml to 10000 pg/ml respectively .
Bioactivity-FACS
Please refer to DS document for the assay protocol.
A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).
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