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Assay Type | FACS |
Analyte | IL-2, IL-4, IL-5, IL-10, TNF-α, IFN-γ |
Format | 50T |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | IL-2 (0.25 pg/mL), IL-4 (0.32 pg/mL), IL-5 (1.15 pg/mL), IL-10 (0.1 pg/mL), TNF-α (0.78 pg/mL), IFN-γ (0.29 pg/mL) |
Standard Curve Range | 9.8-10000 pg/mL (TNF-α, IFN-γ), 9.8-5000 pg/mL (IL-2, IL-4, IL-5, IL-10) |
Assay Time | 3 hr |
Suitable Sample Type | Human serum, Human plasma, Cell culture supernatants |
Sample volume | 30 μL |
T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized-some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.
It is for research use only.
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Catalog | Product Name | Size |
FMBc007-02 | Anti-human IL-2 Antibody-coupled APC-beads-D01 | 50 tests |
IL2-PFS426 | PE-Labeled Monoclonal Anti-human IL-2 Antibody, Rabbit IgG | 50 tests |
FMBc008-02 | Anti-human IL-4 Antibody-coupled APC-beads-D05 | 50 tests |
IL4-PFS269 | PE-Labeled Monoclonal Anti-human IL-4 Antibody, IgG1 | 50 tests |
FMBc014-02 | Anti-human IL-5 Antibody-coupled APC-beads-C03 | 50 tests |
FABm030-01 | PE-Labeled Monoclonal Anti-Human IL-5 Antibody, Mouse Fc (7G2A7) | 50 tests |
FMBc017-02 | Anti-human IL-10 Antibody-coupled APC-beads-D08 | 50 tests |
IL0-PFS428 | PE-Labeled Monoclonal Anti-IL10 Antibody, Rat IgG2a (JES3-12G8) | 50 tests |
FMBc010-02 | Anti-human TNF-α Antibody-coupled APC-beads-D13 | 50 tests |
TNA-PFM494 | PE-Labeled Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) | 50 tests |
FMBc011-02 | Anti-human IFN-γ Antibody-coupled APC-beads-D17 | 50 tests |
IFN-PFM412 | PE-Labeled Monoclonal Anti-IFN-gamma Antibody, human IgG1 (8C5F8) | 50 tests |
FMBf001-02 | APC Positive Control-01 | 0.5 mL |
FMBf001-01 | PE Positive Control-01 | 0.5 mL |
FCMP-02 | 96-well Sealing film | 1 plate |
FCMP-01 | 96-Well V-Bottom Assay Plate | 2 pieces |
FCMB-03 | 1* Assay Buffer | 50 mL |
FCMB-04 | 1*Wash Buffer | 50 mL |
IL2-H51P3 | Calibrator (IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ) | 5 μg for each |
IL5-H52H3 | Calibrator (IL-5) | 50μg |
The Human Th1/Th2 Cytokine Kit-2 is a multiplex bead-based indirect immunofluo-rescence assay, utilizing flow cytometry for data acquisition and classification. The beads conjugated with specific antibody, target protein (IL-2/4/5/10, TNF-α, IFN-γ) in specimens and PE-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. The intensity of PE fluorescence signal in proportion to the target protein in specimens, are assessed by flow cytometry. The present assay has been optimized for quantitatively analysis for the IL-2/4/5/10, TNF-α, IFN-γ in cell culture supernatant, plasma, and serum samples.