Anti-MMAF Antibody Screening Panel

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Cat. No. / Size
Price
Qty
APA-02-80ug
$440.00
ETA of in-stock products:2 business days

Product Details

  • Product Details

    This antibody panel comprises four distinct rabbit monoclonal antibodies against MMAF, with slight differences in their recognition epitopes. Consequently, they may exhibit varying affinity levels towards different MMAF analogs or different conjugates of MMAE and linkers. This panel can be used to conduct preliminary experiments for screening the optimal antibody clone.

  • Components

    Cat.No.
    Clone
    Size
    MMF-MY2213
    1M1G10
    20 ug
    MMF-MY2220
    M1B12
    20 ug
    MMF-MY2214
    1M1E12
    20 ug
    MMF-MY2219
    M1E04
    20 ug
  • Specificity

    Specifically recognizes MMAF and does not recognize MMAE.

  • Source

    This panel contains four anti-MMAF monoclonal antibodies, suitable for pharmacokinetic (PK) detection of ADC with MMAF as the payload. All the anti-MMAF monoclonal antibodies are recombinantly expressed from HEK293, can be used as a capture antibody for detecting conjugated antibodies in ELISA assay.

  • Isotype

    Rabbit IgG | Rabbit Kappa

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

    • Bioactivity-ELISA

       MMAF ELISA

      Immobilized Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (M1E04) (Cat. No. MMF-MY2219) at 1 μg/mL (100 μL/well) can bind IgG1-MMAF with a linear range of 0.1-4 ng/mL (QC tested).

      Protocol
    •  MMAF ELISA

      Immobilized IgG1-MMAF at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. MMF-MY2214) with a linear range of 0.06-0.97 ng/mL (QC tested).

      Protocol
    •  MMAF ELISA

      Immobilized IgG1-MMAF at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1G10) (Cat. No. MMF-MY2213) with a linear range of 0.1-8 ng/mL (QC tested).

      Protocol
    •  MMAF ELISA

      Immobilized Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (M1B12) (Cat. No. MMF-MY2220) at 1 μg/mL (100 μL/well) can bind IgG1-MMAF with a linear range of 0.06-31 ng/mL (QC tested).

      Protocol

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Background

Monomethyl auristatin F (MMAF) is a potent mitotic inhibitor that inhibits tubulin polymerization. MMAF is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAF antibody is a rabbit monoclonal antibody specifically reacts with MMAF without MMAE, which is more sensitive than mouse antibody. The anti-MMAF antibody is a useful reagent in PK assay to determine conjugated antibodies.

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