Human 2B4 / CD244 / SLAMF4 Protein, His Tag

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Cat. No. / Size
Price
Qty
2B4-H5224-100ug
$360.00
2B4-H5224-1mg (500ug X 2)
$2555.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4

  • Source

    Human 2B4, His Tag (2B4-H5224) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # Q9BZW8-2).

    Predicted N-terminus: Cys 22

    Request for sequence
  • Molecular Characterization

    2B4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.1 kDa. The protein migrates as 35-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    2B4 SDS-PAGE

    Human 2B4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     2B4 ELISA

    Immobilized Human 2B4, His Tag (Cat. No. 2B4-H5224) at 5 μg/mL (100 μL/well) can bind Human CD48, Fc Tag (Cat. No. BC1-H5255) with a linear range of 0.078-1.25 μg/mL (QC tested).

    Protocol

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Background

Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.

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