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Product Details
Synonym
Activin A, INHBA
Source
Human Activin A Protein, premium grade (ACA-H5314) is expressed from CHO cells. It contains AA Gly 311 - Ser 426 (Accession # P08476).
Predicted N-terminus: Gly 311
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
GMP-ACAH37 is the GMP version of this ACA-H5314. These two proteins display indistinguishable performance profiles, thereby ensuring a seamless transition for end users from early preclinical stag to later clinical phases.Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 13.0 kDa. The protein migrates as 13 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Activin A Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-CELL BASE
Human Activin A Protein, premium grade (Cat. No. ACA-H5314) stimulates Human Activin RIl (Luc) HEK293 Reporter Cell. The specific activity of Human Activin A Protein, premium grade is＞5.00 x 10^2 IU/mg, which is calibrated against WHO Reference Reagent Activin A (Human, Recombinant) NIBSC code: 91/626 (QC tested).
Protocol
Bioactivity-ELISA
Immobilized Human Activin A Protein, premium grade (Cat. No. ACA-H5314) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, His,Avitag (Cat. No. ACB-H82E3) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Human Activin A Protein, premium grade (Cat. No. ACA-H5314) at 5 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. ACA-H5269) with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
Application Data
Human Activin A Protein, premium grade (Cat. No. ACA-H5314) could effectively induce the endoderm differentiation by FOXA2 and SOX17 expression in immunofluorescence and FACS.
Bioactivity-Stability
The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG Activin A.
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