Human AGO1 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
AG1-H5543-100ug
$380.00
AG1-H5543-1mg
$2600.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    EIF2C, EIF2C1, GERP95, Q99, hAgo1

  • Source

    Human AGO1 Protein, His Tag (AG1-H5543) is expressed from Baculovirus-Insect cells. It contains AA Glu 2 - Ala 857 (Accession # Q9UL18-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    AGO1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 99.1 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    AGO1 SDS-PAGE

    Human AGO1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    AGO1 SEC-MALS

    The purity of Human AGO1 Protein, His Tag (Cat. No. AG1-H5543) is more than 90% and the molecular weight of this protein is around 85-115 kDa verified by SEC-MALS.

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Background

AGO1 (Argonaute-1) is a key protein in the RNA interference (RNAi) pathway. As a core component of the RNA-induced silencing complex (RISC), it binds to small non-coding RNAs like microRNAs (miRNAs) and short interfering RNAs (siRNAs). Unlike its close relative AGO2, AGO1 lacks the slicer activity to cleave target messenger RNAs (mRNAs). Instead, its primary function is to guide RISC to target mRNAs, leading to translational repression and subsequent mRNA degradation. This makes AGO1 crucial for gene silencing and the post-transcriptional regulation of gene expression.

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