Mouse Angiopoietin-like 3 / ANGPTL3 Protein, His Tag

Synonyms

ANGPTL3, ANGPT5, ANG-5, Angiopoietin-5, FHBL2

Source

Mouse Angiopoietin-like 3, His Tag (AN3-M52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 17 - Thr 455 (Accession # Q9R182-1).

Predicted N-terminus: Ser 17

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 221-RAPR-224, and will be partially processed into N and C-terminal fragment with calculated MW of 23.8 kDa and 28.8 kDa respectively. The protein migrates as 60-70 kDa (full lengh), 35-43 kDa (C-terminal fragment) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 0.05% CHAPS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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