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Human Apolipoprotein H Protein, His Tag (MALS verified)

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APH-H52H3-100ug
$310.00
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APH-H52H3-1mg
$2,040.00
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APH-H52H3
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

APOH, Apo-H, B2GPI, Beta(2)GPI, B2G1

Source

Human Apolipoprotein H Protein, His Tag (APH-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Cys 345 (Accession # P02749).

Predicted N-terminus: Gly 20

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 38.1 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Autoantibodies directed to negatively charged phospholipids, in particular cardiolipin, have been detected in the serum of patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). APS is characterised by venous and arterial thrombosis, recurrent spontaneous abortions and thrombocytopenia. It is now known that a serum cofactor, β2-glycoprotein 1, is required for the binding of cardiolipin by autoantibodies in the sera of patients with APS. By contrast, anticardiolipin antibodies from patients with infectious diseases (in particular, syphilis) do not require this cofactor.
β2-Glycoprotein 1, also known as apolipoprotein H, is a relatively abundant serum protein (present at a concentration of about 0.2 mg/ml) that may play a role in coagulation. It has been shown to bind to platelets, mitochondria and negatively charged substances such as heparin, D, dextran sulphate and negatively charged phospholipids.