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Product Details
Synonyms
APOH, Apo-H, B2GPI, Beta(2)GPI, B2G1
Source
Human Apolipoprotein H Protein, His Tag (APH-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Cys 345 (Accession # P02749).
Predicted N-terminus: Gly 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 38.1 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Apolipoprotein H Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Apolipoprotein H Protein, His Tag (Cat. No. APH-H52H3) is more than 95% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human Apolipoprotein H Protein, His Tag (Cat. No. APH-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LDL R, His,Avitag (Cat. No. LDR-H82E7) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
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Background
β2-Glycoprotein 1, also known as apolipoprotein H, is a relatively abundant serum protein (present at a concentration of about 0.2 mg/ml) that may play a role in coagulation. It has been shown to bind to platelets, mitochondria and negatively charged substances such as heparin, DNA, dextran sulphate and negatively charged phospholipids.
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