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Biotinylated Cynomolgus Carbonic Anhydrase XII Protein, His,Avitag™ (active enzyme, MALS verified)

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CAI-C82E3-20ug
$310.00
In Stock - Delivery in 1-2 business days
CAI-C82E3-100ug
$905.00
In Stock - Delivery in 1-2 business days
CAI-C82E3-500ug (250ug X 2)
$3,090.00
In Stock - Delivery in 2 weeks
CAI-C82E3
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

Carbonic Anhydrase XII, CA XII, Car12, CA-XII, Carbonate dehydratase XII

Source

Biotinylated Cynomolgus Carbonic Anhydrase XII Protein, His,Avitag (CAI-C82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Gly 299 (Accession # A0A2K5TSB2-1).

Predicted N-terminus: Ala 25

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 34.5 kDa. The protein migrates as 42-52 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50mM Tris,150mM NaCl,PH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.