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CD133, PROM1, PROML1, Prominin-1, AC133
Nanodisc Human CD133 Full Length Protein, His Tag (CD3-H52H1) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - His 865 (Accession # O43490-1).
Predicted N-terminus: Gly 20
The CD133 carries a polyhistidine tag at the C-terminus with calculated MW of 97.2 kDa and migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
>90% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.